This is the ENCODE-DCC RNA-sequencing pipeline. The scope of the pipeline is to align reads, generate signal tracks, and quantify genes and isoforms.
RNA-seq data is valuable as it allows the measure of RNA expression levels as a transcriptional readout and the study of RNA structures in order to understand how RNA-based mechanisms impact gene regulation and thus disease and phenotypic variation.
Costs for this workflow typically vary between $10-$25 per job and depends mostly on the size and number of replicates.
|Public example||Job cost||Notes|
|ENCSR954PZB total RNA-seq on human adrenal gland||$11.25||This is a paired end sample with 2 replicates. Each of the 4 fastq files is around 4GB. Rerun of ENCODE experiment ENCSR954PZB|
|PVTT_RNA_seq||$11.53||This is a paired end sample with 2 replicates.|
*Job cost examples are for estimates only. To get a more accurate idea of job costs, try running a single job before running many jobs.
- Encode Project Website